Groundwater crustacean distribution mapping from Spain, the Balearic and Canary Islands

The current estimation of groundwater crustaceans from Spain, the Balearic and the Canarian Islands accounts for more than 250 species of crustaceans known from karst and porous aquifers. More than 70 species are endemics, however several species found especially from caves are new to science and endemics. The hotspot of biodiversity is registered in northern part of Spain in Cantabria and the Basque Country. The Balearic Islands has particular subterranean crustaceans wih several species of marine origin. Still in several regions basic informations on biodiversity and species richness are lacking such is Galicia, Extremadura and large parts of Andalusia running the risk that an undocumented biodiversity would be affected by hydrological changes ocuring in several aquifers, due on one side to human impact (overexploitation for urban and agricultural purposes) and climatic changes (that reduce the groundwater recharge).
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Groundwater crustaceans mapping from Spain, the Balearic and Canary Islands in caves, hyporheic zone, aquifers and springs habitats

Spain is one of the most affected country from southern Europe by droughts. Climate change projections forecast an increase of temperature, decrease of precipitation, and increased occurrence of extreme events that would influence the groundwater recharge, its quantity and quality. Spain has also a huge groundwater demand for urban and agriculture acitivities and tourism. These double impacts on groundwater systems would lead to a loss of biodiversity underground sometimes even before the species being discovered. Without prior surveys of groundwater to know what species are present it is imposible to estimate the impacts (Work in progress).

Implementation of new technologies in the field of agriculture: SMART-HYDRO

SMART-HYDRO  – Intelligent system to optimise the use of water in agriculture, proposes a new research approach based on information technologies and sensors. Smart-Hydro is a real-time, decision making support system that enables the water needs of each crop to be determined and optimises water resource use and management.

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General vision of SMART-HYDRO concept

Historically, monitoring of crop status and needs has been based on traditional techniques associated with direct observation of crops, the land and experience. In the same way that the digital revolution has transformed our everyday lives and the way we interact with our environment, the arrival of new information and communication technologies (ICT) to sectors like agriculture represents a breakthrough in terms of productivity and a reduction in environmental impact. The introduction of ICT into the equation opens up a wide range of possibilities in agriculture and forms the basis for raising awareness of environmental issues and the importance of reasonable use of water. Collaboration between research centres and technology companies in the Smart-Hydro project is facilitating the development of an advanced support tool for decision making and water management in agriculture, based on the needs of the crop and associated environmental factors. This support tool is based on the new possibilities offered by ICT. Part of the work in the project has involved the sensorisation of two pilot plots on which different crops (potatoes and corn) are cultivated for the purpose of validation and demonstrating how the tool operates.

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Aerial images taken by drones to study the vegetative state of the crops

A battery of sensors is installed on each of these plots for wireless transmission of data using the GPRS (General Packet Radio Service) system. This data provides information on soil conditions and forecasts on crop water needs in real time. Another innovative element of Smart-Hydro is the use of UAV (Unmanned Aerial Vehicles) or drones to study and monitor the vegetative state of the crop. The treatment and interpretation of the images obtained by infrared and multispectral (wavelengths of 530nm, 550nm, 570nm, 670nm, 700nm and 800nm) cameras installed on the UAV enables the calculation of a number of vegetation indexes for the purpose of determining the phenological status of the crop. Such indexes include the EVI (Enhanced Vegetation Index), NDVI (Normalized Difference Vegetation Index) and SAVI (Soil Adjusted Vegetation Index). These indexes use the radiometric behaviour of the vegetation, mainly in the visible and near infrared spectrums, to determine which vegetation is growing healthily and thriving and which is diseased or growing with low density. Such aspects are not easy to identify in large plantations or with the naked eye. All this data, along with climate variables obtained with weather stations, is integrated on a platform, where it is processed along with the images obtained by the UAVs. The technologies implemented in Smart-Hydro provide information on the water requirements of a crop, based mainly on temperature and atmospheric humidity, rainfall, incident solar radiation, vegetative status of plants and quantity of water available in the soil.

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Evolution of the vegetation through the study of the images captured by the drones with the multispectral and thermal camera

In addition to water needs, all plantations have an associated environmental impact that must be assessed and quantified, given that it is an additional externality of the production process. This indicator is called water footprint, which basically includes water from precipitation, irrigation water from surface or groundwater sources, and the water needed to dilute fertilizers and/or pesticides to concentrations that are acceptable for the natural environment. The ultimate objective of Smart-Hydro is, based on this real-time information, to provide users from any web platform with access to an application with a series of functions, such as the “Servicio de Asesoramiento al Riego (Irrigation Advisory Service – SAR)”, the carbon and water footprints associated with a crop after harvesting, and the Water Stress Index (WSI) of the vegetation. To make the design model as real as possible, it is necessary to characterize the natural environment in which the pilot plots are located and study the factors involved in growth of the vegetation. A crop demands water, nutrients, and sunlight to grow healthily and productively. If any of these elements does not meet the requirements of plants, then their vegetative and reproductive cycles will be affected, with consequent adverse effects on harvest results. To make the design model as real as possible, it is necessary to characterise the natural environment in which the pilot plots are located and study the factors involved in growth of the vegetation. A crop demands water, nutrients, and sunlight to grow healthily and productively. If any of these elements does not meet the requirements of plants, then their vegetative and reproductive cycles will be affected, with consequent adverse effects on harvest results.

By way of example

The irrigation of a crop of this type (corn or potato) is a closed cycle involving the extraction of water with certain physicochemical and quality characteristics from the aquifer or reservoir (reservoir, lake or river). This water is used water for irrigation and the surplus irrigation water, with modified physicochemical and quality characteristics, is returned to the subsoil or river, by infiltration or runoff. Traditional agriculture uses supplements and/or fertilisers to improve vegetable growth, as well as plant protection products to prevent or eliminate plagues. These chemical compounds, some of which are very persistent in the environment, are carried by the surplus irrigation water and build up in ecosystems and the fauna associated with these ecosystems.

In order to include these factors in the equation, the SMART-HYDRO project team is undertaking a hydrogeochemical study of the groundwater body (GB) from which the irrigation water for the pilot plots is extracted, called “Aluvial Aquifers: Jarama-Tajuña (030.007)”; and of the surface water bodies (Jarama River and lakes) in the surrounding areas that are connected with the river-aquifer system of the area. If the initial quantitative and qualitative characteristics of the water resources and the fauna that inhabit the ecosystems associated with the GB (which serve as bio-indicators) are known, variations in initial conditions and trends over time can be monitored, and assessment can be made as to whether the crops cultivated on the surface have a significant effect. Characterisation and monitoring of the state of water bodies is important in order to plan sustainable management of the water resource in a manner that is compatible with the real needs of the crop.

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Groundwater fauna sampling

SMART-HYDRO– Intelligent system to optimise the use of water in agriculture, is an experimental project in which know-how and techniques from different areas are combined from a scientific and technological perspective. The SMART-HYDRO consortium is made up of 6 partners: AIN, IMDEA Agua, Inkoa, Innovati, Neiker and Sensing & Control.

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The God, the Bad and the Ugly about flame retardants – the hidden effect of wildfire on aquatic ecosystems

The wildfire occurring in the past weeks in Pedrógão Grande, eastern Portugal and near Doñana National Park in southern Spain, ended with an enormous lives lost (64 people in Portugal) and the evacuation of more than 10,000 people from the surrounding areas. The fires affected 30,000 hectares in Portugal and almost 10,000 ha in Spain. Hundreds of firefighters and volunteers worked to combat the blaze and the flames that overwhelmed the trees for days. In both sites the fires seems to be the results of “forest management errors and bad political decisions” by governments in the recent decades. In the special case of Doñana, a UNESCO World Heritage Site and a Biosphere Reserve, WWF Spain claimed from several years the hazard of forest fires due to the uncontrolled extent of public forest for different uses (www.). Between 2005 and 2009, WWF Spain signaled the presence of a total of 80 fires in the Doñana area that affect the wildlife in one the most extended coastal wetland from southern Europe.

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Figure 1. Wildfire in Doñana National Park (source: El Pais)

Forest fires usually occur in nature and play an essential ecological role in maintaining the ecosystem health, contributing to forests regeneration, stimulation of seed germination and the returning of important nutrients to the forest soil previously stored in biomass. After a natural forest fire, starts a process of ecological succession where the ecosystems pass it to several changes and develop in a mature forest again. This means a recolonization of the soil with herbaceous species, grasses and weeds, followed by taller plants and the last comers are tree species. Natural wildfires also influence the hydrological cycle immediately after post-fire period as well as the water quality at the surface and underground.

The most evident changes in surface waters post-fire are sediments loads particularly in suspended fraction in streams that affect temporary the biological habitats available for aquatic organisms from the streams channel and the riparian zone, such are fishes and benthic and interstitial invertebrates. The changes in water chemical properties after a wildfire are not well documented, but the studies suggest an increase of nutrients loads, and especially of phosphorus and nitrogen. An estimation of the nitrogen content in a wildfire from a forest experimental area in California suggest that the nitrogen might be 10 times higher post-fires events and its concentration remains higher even after three years (Westerling & Bryant, 2008). However, the increment of nutrient loads after a fire event highly depends by the watershed, type of forest and local/regional climatic conditions. Groundwater in forested areas is usually pristine, since the soil forest significantly contributes to water purification. The primary source of groundwater contamination after a fire, results from microorganisms that can enter underground due to the soil destruction, sediments loads, ash and potential changes in water pH. Post-fire there is a high alkalinity runoff from burned areas that may increase temporary the pH in both surface and groundwater, but this can be neutralized if it is diluted in sufficient quantities of water.

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Figure 2. Tundra wildfire burn in Alaska on June 7, 2005 (Source: Matt Snyder—Alaska Division of Forestry/AP)

On medium and long term wildfires are beneficial for the ecosystems, either terrestrial (vegetation) or aquatic (surface and groundwater). However, an important aspect resulted from the wildfires is the contamination with fire extinguishing agents. These chemical substances called flame retardants are used worldwide since the beginning of the 1970. Flame retardants are chemical compounds that suppress the flames and are used as a prevention measures or when a fire is already occurring. Airplanes also release flame retardants into the forests, and they are usually applied as prevention in well-known areas susceptible to fires, or when the fire is already occurring. They are able to alter the combustion even after the water is removed by evaporation during burning.

Flame retardants consist in a mixture of water (85%), inorganic chemicals (10%) and coloring agents and stabilizers (5%). The most common inorganic chemicals used nowadays are based on ammonia (phosphates and sulphates of ammonia) or brome. In the past, all retardants contained sodium ferrocyanide as a corrosion inhibitor, but due to their toxicity especially UV irradiation they were banned since 2007. Flame retardants are also present around us in plastics, textiles, electronic circuitry and other materials to prevent fires.

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Figure 3. Drops of flame retardants on a wildfire

The retardants way of action is complex and depends by the specific nature of the material they are protecting and are made off. For example, the ammonium adheres to the surface of vegetation and can retard the flame, the phosphates can react with some of the active species appeared after combustion and inhibit the propagation of flames, whereas the brominated flame retardants release bromine atoms (free radicals) into the gas phase and reduce the heat generated and slow or prevent the burning process.       

Flame retardants and water contamination In the last decade there has been an intense debate about the risk that pose the flame retardants on environment, in general and on aquatic ecosystems, in particular. At the beginning the negative impact on environment of flame retardants was neglected, as their main active ingredients are agricultural fertilizers. However, a compound even if it has low toxicity can cause adverse environmental effects, when there is an intensive use of the product and it is accumulating in water, soil, atmosphere from where they are finally uptake by organisms. The nowadays concern about these contaminants is justified since several of them are corrosive and toxic, have limited biodegradability (as all halogenated organic compounds) being hence highly persistent and tend to accumulate in the environment on long term. The bioaccumulation of flame retardants as any other persistent “anthropogenic” compound is notable in the food chain, being found in zooplankton, invertebrates and fishes (Bayen et al., 2008; Segev et al., 2009). Flame retardants appear not only to be the attribute of human abilities to synthetize chemically these compounds, but can be also produced in nature by organisms. Recently, a group of researchers from the Scripps Institution of Oceanography at the University of California San Diego found a marine sponge that host a bacteria able to produce toxic compounds nearly identical to human-made fire retardants (Agarwal et al., 2017).

Toxicity effect of flame retardants on aquatic organisms The toxicity of flame retardants components on aquatic organisms are due to their inorganic and organic components. Hence, ammonium salts is one of the most toxic compound when it is dissociated to ammonia (a common compound resulted from nitrates reduction by bacteria). The effects of the ammonia further depend by the water temperature and pH since they influence the equilibrium reaction of ammonium-ammonia. Toxicity studies with flame retardants have been mainly performed in laboratory conditions on algae, amphibians and fishes, the later in different development stages (Hamilton et al. 1996; Buhl and Hamilton 1998). These laboratory testing aiming to tests the fishes mortality are of huge help because dose-response studies have significant implications on simulation models developed to estimate the effect of direct delivery of flame retardants on water and organisms mortality. An important direct effect of flame retardants toxicity was detected to be enhanced in the presence the UV-B radiation. The toxicity of the retardant in this case is due to sodium ferrocyanide, a corrosion inhibitor that is toxic for the fish Oncorhynchus mykiss and the frog Rana sphenocephala because it releases cyanides (Little and Calfee 2000). Brominated flame retardants have high acute toxicity to aquatic organisms, such as algae, mollusks, crustaceans and fish and at the cellular level their action has been proved to induce hemocyte lysosomal membrane destabilization (Canesi et al., 2005).

The outdoor testing of flame retardants on fish’s shows that these compounds even when highly diluted is lethal for aquatic organisms (Gaikowski et al. 1996). Changes in the concentration of retardants in streams were detected up to 2.7 km downstream the aerial release and mortality was registered after 1 day. The spills of the flame retardants in streams cause substantial mortality of fishes but this also depends by the stream size, flow rates and type of interstitial riverbed sediments and soil. The soil type and instream sediments have a significant implication on the retention of flame retardants, since there are ions exchanges among soil and the component substances of the retardant. The ash resulted from the fire, at low concentration clogs the fish’s gills and imped the respiration, these having sometimes a similar hazardous effect as the chemical compounds from the retardants.

The side effects of fire-fighting chemicals on wildlife in general and on aquatic organisms in particular are inherent, and it should be considered by fire control managers to protect the biota and their aquatic habitats. Bioremediation process post-fires are the most significant action that managers could take into account rapidly as to eliminate or at least to reduce the flame retardants compounds from the “receiving” environment. Although the bioremediation processes are difficult and complex, the recent studies on biodegradation of flame retardants using microorganisms in both aerobic and anaerobic conditions are promising (Segev et al., 2008). Among them the most effective are dehalogenating bacteria present in groundwater that has been proved to biodegrade during transport in low permeability chalk aquifer (Amon et al., 2005). In the case of in situ groundwater bioremediation, several particularities of the aquifer should be considered such are the physicochemical conditions of the rock and water, residence time of the water and biological factors. Several bioremediation tests have been also performed in reactors with controlled conditions and concentrated biomass acclimated to treat the industrial wastewaters using biological treatment. These test indicates a significant biodegradation of the flame retardant 2,4,6-tribromophenol (TBP) in aerobic conditions. TBP was also conceivable to be biodegraded in anaerobic conditions by bacteria such as Achromobacter piechaudii, Desulfovibrio stain TBP-1 and Ochrobacterium sp. strain TB01.

Since the effects of several flame retardants compound on surface and groundwater aquatic invertebrates is poorly known, the Groundwater Ecology Group from IMDEA Agua aim to design a protocol for testing, evaluating and determination of the sensitivity of surface and typical groundwater invertebrate species to flame retardants compounds recently detected in Spanish waters. We specifically aim to tackle the lethal and sublethal concentrations of selected flame retardant contaminants on surface and groundwater crustacean invertebrates. Our study targets to contribute to current attempts in establishing the effects of flame retardant compounds on aquatic organisms and to advance the assessment ecological risk tools especially in one of the most vulnerable aquatic ecosystem and challenging in terms of remediation – the groundwater. 

Reference

Arnon S., A. Eilon, Z. Ronen, A. Nejidat, A. Yakirevich, R. Nativ. 2005. Biodegradation of 2,4,6-Tribromophenol during transport in fractured chalk. Environ. Sci. Technol. 39: 748–755.

Agarwal, V., J.M. Blanton, S. Podell, A. Taton, M.A. Schorn, J. Busch, Z. Lin, E.W. Schmidt, P.R. Jensen, V.J. Paul, J.S. Biggs, J.W. Golden, E.E. Allen, B.S. Moore. Metagenomic discovery of polybrominated diphenyl ether biosynthesis by marine sponges. Nature Chemical Biology, 2017; 13 (5): 537.

Canesi L, Lorusso LC, Ciacci C, Betti M, Gallo G., 2005. Effects of the brominated flame retardant tetrabromobisphenol-A (TBBPA) on cell signaling and function of Mytilus hemocytes: involvement of MAP kinases and protein kinase C. Aquat Toxicol. 75(3):277-87.

Westerling, A.L. & Bryant, B.P. 2008. Climatic Change. 87 (1): 231.